Attorney General Pam Bondi announced the legal action at a news conference in Washington on Wednesday.

She appeared alongside former University of Kentucky swimmer Riley Gaines, who has emerged as a public face of the opposition to transgender athletes.

Trump’s departments of Education and Health and Human Services have said the Maine agency is violating the federal Title IX anti-discrimination law by allowing transgender girls to participate on girls’ teams.

Maine officials have refused to agree with a settlement that would have banned transgender students from sports, arguing that the law does not prevent schools from letting transgender athletes participate.